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pbs odyssey blocking buffer  (LI-COR)


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    LI-COR pbs odyssey blocking buffer
    Pbs Odyssey Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+odyssey+blocking+buffer/Intercept+(PBS)+Blocking+Buffer/bio_rxiv__64898__2026__05__08__723929-181-10-15
    Average 99 stars, based on 653 article reviews
    pbs odyssey blocking buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: DNA antibody constructs encoding anti-ZIKV envelope antibodies
    Article Snippet: The proteins were transferred onto nitrocellulose membranes using the iBlot 2 Gel Transfer Device (Life Technologies). .. The membranes were blocked in PBS Odyssey blocking buffer (LI-COR Biosciences) for 1 h at room temperature. .. To detect vaccine expression, the anti-Flavivirus group antigen (MAB10216-Clone D1-4G2-4-15) antibody was diluted 1:500 and the immune serum from mice and RM was diluted 1:50 in Odyssey blocking buffer with 0.2% Tween 20 (Bio-Rad) and incubated with the membranes overnight at 4° C. The membranes were washed with PBST and then incubated with the appropriate secondary antibody (goat anti-mouse IRDye680CW; LI-COR Biosciences) for mouse serum and flavivirus antibody; and goat anti-human IRDye800CW (LI-COR Biosciences) for RM sera at 1:15,000 dilution for mouse sera for 1 h at room temperature.

    Article Title: Neonatal Procedural Pain Disrupts Phosphorylation of KCC2 in the Spinal Cord
    Article Snippet: Blots were incubated for 1 h with Odyssey blocking buffer (Li‐Cor, Lincoln, NE, USA). .. For KCC2 quantification, blots were incubated overnight at 4°C with rabbit anti‐KCC2 antibody (1:1000, Sigma, lot#3872327) and mouse anti‐GAPDH (1:1,000,0000, Thermo Fisher), diluted in 1:1 PBS:Odyssey blocking buffer (Li‐Cor). .. The next day, blots were washed for 3 × 5 min with PBS containing 0.01% Tween (PBS‐T) followed by 1‐hour incubation with secondary antibodies, including goat anti‐rabbit IRDye 800 (1:10,000, Li‐Cor) and donkey anti‐mouse IRDye 600 (1:10,000, Li‐Cor).

    Article Title: DNA antibody constructs encoding anti-ZIKV envelope antibodies
    Article Snippet: The proteins were transferred onto nitrocellulose membranes using the iBlot 2 Gel Transfer Device (Life Technologies). .. The membranes were blocked in PBS Odyssey blocking buffer (LI-COR Biosciences) for 1 hour at room temperature. .. The anti-Flavivirus group antigen (MAB10216-Clone D1-4G2-4-15) antibody was diluted 1:500 to detect vaccine expression and the immune serum from mice and RM was diluted 1:50 in Odyssey blocking buffer with 0.2% Tween 20 (Bio-Rad) and incubated with the membranes overnight at 4° C. The membranes were washed with PBST and then incubated with the appropriate secondary antibody [Goat anti-mouse IRDye680CW (LICOR) for mouse serum and flavivirus antibody; and Goat anti-human IRDye800CW (LICOR) for RM Sera] at 1:15,000 dilution for mouse sera for 1 hour at room temperature.

    Article Title: The Effects of Ingesting a Single Bolus of Hydrolyzed Collagen versus Free Amino Acids on Muscle Connective Protein Synthesis Rates
    Article Snippet: .. Samples were run on a Criterion Precast TGX 4–15% gel (Order No. 567–8085; Bio‐Rad) ± 90 min at 150 V (constant voltage) and transferred onto a Trans‐blot Turbo 0.2 μm nitrocellulose membrane (Order No. 170– 4159; Bio‐Rad) in 10 min at 2.5 A and 25 V. Specific proteins were detected by overnight incubation at 4°C on a shaker with specific antibodies in 50% in PBS Odyssey blocking buffer (Part No. 927‐40 000; Li‐Cor Biosciences, Lincoln, NE, USA) after blocking for 60 min at RT in 50% in PBS Odyssey blocking buffer. .. Primary antibodies used were: Pro-Collagen SP1.D8 (Developmental Studies Hybridoma Bank, Iowa City, IA, 1:500), phosphor-eEF2 (Thr 56) (#2331, 1:1000), phosphor-ribosomal S6 (Ser 240/244) (#5364, 1:3000), phosphor-P44/42 MAPK (ERK 1/2) (Thr 202/Tyr 204) (#4370, Cell Signaling Danvers, MS, 1:1500), MMP8 (sc-514803, 1:500) and MMP1 (sc-58377, Santa Cruz Biotechnology, Dallas, TX, 1:2500).

    Article Title: Vaccines against Zika virus
    Article Snippet: .. The membranes were blocked in PBS Odyssey blocking buffer (LI-COR Biosciences) for 1 h at room temperature. .. To detect vaccine expression, the anti-Flavivirus group antigen (MAB10216-Clone D1-4G2-4-15) antibody was diluted 1:500 and the immune serum from mice and RM was diluted 1:50 in Odyssey blocking buffer with 0.2% Tween 20 (Bio-Rad) and incubated with the membranes overnight at 4° C. The membranes were washed with PBST and then incubated with the appropriate secondary antibody (goat anti-mouse IRDye680CW; LI-COR Biosciences) for mouse serum and flavivirus antibody; and goat anti-human IRDye800CW (LI-COR Biosciences) for RM sera at 1:15,000 dilution for mouse sera for 1 h at room temperature.

    Article Title: The Effects of Ingesting a Single Bolus of Hydrolyzed Collagen versus Free Amino Acids on Muscle Connective Protein Synthesis Rates
    Article Snippet: .. Samples were run on a Criterion Precast TGX 4%–15% gel (order no. 567-8085; Bio-Rad) ± 90 min at 150 V (constant voltage) and transferred onto a Trans-blot Turbo 0.2 μm nitrocellulose membrane (order no. 170-4159; Bio-Rad) in 10 min at 2.5 A and 25 V. Specific proteins were detected by overnight incubation at 4°C on a shaker with specific antibodies in 50% in PBS Odyssey blocking buffer (part no. 927-40 000; Li-Cor Biosciences, Lincoln, NE) after blocking for 60 min at RT in 50% in PBS Odyssey blocking buffer. .. Primary antibodies used were as follows: Pro-Collagen SP1.D8 (Developmental Studies Hybridoma Bank, Iowa City, IA, 1:500), phosphor-eEF2 (Thr 56) (no. 2331, 1:1000), phosphor-ribosomal S6 (Ser 240/244) (no. 5364, 1:3000), phosphor-P44/42 MAPK (ERK 1/2) (Thr 202/Tyr 204) (no. 4370, Cell Signaling Danvers, MS, 1:1500), MMP8 (sc-514803, 1:500), and MMP1 (sc-58377; Santa Cruz Biotechnology, Dallas, TX, 1:2500).

    Article Title: Vaccines against Zika virus
    Article Snippet: The proteins were transferred onto nitrocellulose membranes using the iBlot 2 Gel Transfer Device (Life Technologies). .. The membranes were blocked in PBS Odyssey blocking buffer (LI-COR Biosciences) for 1 hour at room temperature. .. The anti-Flavivirus group antigen (MAB10216-Clone D1-4G2-4-15) antibody was diluted 1:500 to detect vaccine expression and the immune serum from mice and RM was diluted 1:50 in Odyssey blocking buffer with 0.2% Tween 20 (Bio-Rad) and incubated with the membranes overnight at 4° C. The membranes were washed with PBST and then incubated with the appropriate secondary antibody [Goat anti-mouse IRDye680CW (LICOR) for mouse serum and flavivirus antibody; and Goat anti-human IRDye800CW (LICOR) for RM Sera] at 1:15,000 dilution for mouse sera for 1 hour at room temperature.

    Article Title: Glutamine codon-driven translational readthrough reveals context-dependent stop codon decoding fidelity
    Article Snippet: Protein was then transferred to 0.45 μm nitrocellulose membranes ( Bio-Rad ) with cold 1X Trans-Blot Turbo buffer in a semi-dry transfer apparatus (Trans-Blot Turbo Transfer System, Bio-Rad ), or, if protein was over 100 kDa (Cyc8, Snf5), in a wet transfer apparatus for 3 h at 70V. .. Membranes were incubated at room temperature for 1 h in PBS Odyssey Blocking Buffer (927-4000, LI-COR ) and incubated in primary antibody solutions at 4°C overnight. .. Membranes were then washed three times in 1X PBS with 0.1% Tween-20 (PBS-T, 5 min per wash) at room temperature before incubating in secondary antibody solutions at room temperature for 2.5 h. Membranes were washed three times in PBS-T at room temperature prior to imaging with the Odyssey system ( LI-COR Biosciences ).

    Incubation:

    Article Title: Neonatal Procedural Pain Disrupts Phosphorylation of KCC2 in the Spinal Cord
    Article Snippet: Blots were incubated for 1 h with Odyssey blocking buffer (Li‐Cor, Lincoln, NE, USA). .. For KCC2 quantification, blots were incubated overnight at 4°C with rabbit anti‐KCC2 antibody (1:1000, Sigma, lot#3872327) and mouse anti‐GAPDH (1:1,000,0000, Thermo Fisher), diluted in 1:1 PBS:Odyssey blocking buffer (Li‐Cor). .. The next day, blots were washed for 3 × 5 min with PBS containing 0.01% Tween (PBS‐T) followed by 1‐hour incubation with secondary antibodies, including goat anti‐rabbit IRDye 800 (1:10,000, Li‐Cor) and donkey anti‐mouse IRDye 600 (1:10,000, Li‐Cor).

    Article Title: The Effects of Ingesting a Single Bolus of Hydrolyzed Collagen versus Free Amino Acids on Muscle Connective Protein Synthesis Rates
    Article Snippet: .. Samples were run on a Criterion Precast TGX 4–15% gel (Order No. 567–8085; Bio‐Rad) ± 90 min at 150 V (constant voltage) and transferred onto a Trans‐blot Turbo 0.2 μm nitrocellulose membrane (Order No. 170– 4159; Bio‐Rad) in 10 min at 2.5 A and 25 V. Specific proteins were detected by overnight incubation at 4°C on a shaker with specific antibodies in 50% in PBS Odyssey blocking buffer (Part No. 927‐40 000; Li‐Cor Biosciences, Lincoln, NE, USA) after blocking for 60 min at RT in 50% in PBS Odyssey blocking buffer. .. Primary antibodies used were: Pro-Collagen SP1.D8 (Developmental Studies Hybridoma Bank, Iowa City, IA, 1:500), phosphor-eEF2 (Thr 56) (#2331, 1:1000), phosphor-ribosomal S6 (Ser 240/244) (#5364, 1:3000), phosphor-P44/42 MAPK (ERK 1/2) (Thr 202/Tyr 204) (#4370, Cell Signaling Danvers, MS, 1:1500), MMP8 (sc-514803, 1:500) and MMP1 (sc-58377, Santa Cruz Biotechnology, Dallas, TX, 1:2500).

    Article Title: The Effects of Ingesting a Single Bolus of Hydrolyzed Collagen versus Free Amino Acids on Muscle Connective Protein Synthesis Rates
    Article Snippet: .. Samples were run on a Criterion Precast TGX 4%–15% gel (order no. 567-8085; Bio-Rad) ± 90 min at 150 V (constant voltage) and transferred onto a Trans-blot Turbo 0.2 μm nitrocellulose membrane (order no. 170-4159; Bio-Rad) in 10 min at 2.5 A and 25 V. Specific proteins were detected by overnight incubation at 4°C on a shaker with specific antibodies in 50% in PBS Odyssey blocking buffer (part no. 927-40 000; Li-Cor Biosciences, Lincoln, NE) after blocking for 60 min at RT in 50% in PBS Odyssey blocking buffer. .. Primary antibodies used were as follows: Pro-Collagen SP1.D8 (Developmental Studies Hybridoma Bank, Iowa City, IA, 1:500), phosphor-eEF2 (Thr 56) (no. 2331, 1:1000), phosphor-ribosomal S6 (Ser 240/244) (no. 5364, 1:3000), phosphor-P44/42 MAPK (ERK 1/2) (Thr 202/Tyr 204) (no. 4370, Cell Signaling Danvers, MS, 1:1500), MMP8 (sc-514803, 1:500), and MMP1 (sc-58377; Santa Cruz Biotechnology, Dallas, TX, 1:2500).

    Article Title: Glutamine codon-driven translational readthrough reveals context-dependent stop codon decoding fidelity
    Article Snippet: Protein was then transferred to 0.45 μm nitrocellulose membranes ( Bio-Rad ) with cold 1X Trans-Blot Turbo buffer in a semi-dry transfer apparatus (Trans-Blot Turbo Transfer System, Bio-Rad ), or, if protein was over 100 kDa (Cyc8, Snf5), in a wet transfer apparatus for 3 h at 70V. .. Membranes were incubated at room temperature for 1 h in PBS Odyssey Blocking Buffer (927-4000, LI-COR ) and incubated in primary antibody solutions at 4°C overnight. .. Membranes were then washed three times in 1X PBS with 0.1% Tween-20 (PBS-T, 5 min per wash) at room temperature before incubating in secondary antibody solutions at room temperature for 2.5 h. Membranes were washed three times in PBS-T at room temperature prior to imaging with the Odyssey system ( LI-COR Biosciences ).

    Membrane:

    Article Title: The Effects of Ingesting a Single Bolus of Hydrolyzed Collagen versus Free Amino Acids on Muscle Connective Protein Synthesis Rates
    Article Snippet: .. Samples were run on a Criterion Precast TGX 4–15% gel (Order No. 567–8085; Bio‐Rad) ± 90 min at 150 V (constant voltage) and transferred onto a Trans‐blot Turbo 0.2 μm nitrocellulose membrane (Order No. 170– 4159; Bio‐Rad) in 10 min at 2.5 A and 25 V. Specific proteins were detected by overnight incubation at 4°C on a shaker with specific antibodies in 50% in PBS Odyssey blocking buffer (Part No. 927‐40 000; Li‐Cor Biosciences, Lincoln, NE, USA) after blocking for 60 min at RT in 50% in PBS Odyssey blocking buffer. .. Primary antibodies used were: Pro-Collagen SP1.D8 (Developmental Studies Hybridoma Bank, Iowa City, IA, 1:500), phosphor-eEF2 (Thr 56) (#2331, 1:1000), phosphor-ribosomal S6 (Ser 240/244) (#5364, 1:3000), phosphor-P44/42 MAPK (ERK 1/2) (Thr 202/Tyr 204) (#4370, Cell Signaling Danvers, MS, 1:1500), MMP8 (sc-514803, 1:500) and MMP1 (sc-58377, Santa Cruz Biotechnology, Dallas, TX, 1:2500).

    Article Title: The Effects of Ingesting a Single Bolus of Hydrolyzed Collagen versus Free Amino Acids on Muscle Connective Protein Synthesis Rates
    Article Snippet: .. Samples were run on a Criterion Precast TGX 4%–15% gel (order no. 567-8085; Bio-Rad) ± 90 min at 150 V (constant voltage) and transferred onto a Trans-blot Turbo 0.2 μm nitrocellulose membrane (order no. 170-4159; Bio-Rad) in 10 min at 2.5 A and 25 V. Specific proteins were detected by overnight incubation at 4°C on a shaker with specific antibodies in 50% in PBS Odyssey blocking buffer (part no. 927-40 000; Li-Cor Biosciences, Lincoln, NE) after blocking for 60 min at RT in 50% in PBS Odyssey blocking buffer. .. Primary antibodies used were as follows: Pro-Collagen SP1.D8 (Developmental Studies Hybridoma Bank, Iowa City, IA, 1:500), phosphor-eEF2 (Thr 56) (no. 2331, 1:1000), phosphor-ribosomal S6 (Ser 240/244) (no. 5364, 1:3000), phosphor-P44/42 MAPK (ERK 1/2) (Thr 202/Tyr 204) (no. 4370, Cell Signaling Danvers, MS, 1:1500), MMP8 (sc-514803, 1:500), and MMP1 (sc-58377; Santa Cruz Biotechnology, Dallas, TX, 1:2500).



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